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RELATIONSHIP OF THE BACTERIA INHIBITING PLAQUE FORMATION AND THE NUMBER OF ORAL BACTERIA

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Abstract

¼Ò¾ÆÀÇ ±¸°­À¸·ÎºÎÅÍ ºÐ¸®ÇÑ ºñ¼ö¿ë¼º ±Û·çĵ Çü¼º ¾ïÁ¦¼¼±ÕÀÇ Streptococcus mutans Ä¡Å Çü¼º¿¡ ´ëÇÑ ¾ïÁ¦ Á¤µµ¿Í ºÐ¸® ¼¼±Õ¼ÓÀÇ Å¸¾×³» ³óµµ°¡ Ÿ¾×³» Àüü ¼¼±ÕÀÇ ³óµµ¿¡ ¹ÌÄ¡´Â ¿µÇâÀ» º» °á°ú ´ÙÀ½°ú °°Àº °á°ú¸¦ ¾ò¾ú´Ù.
±¸°­À¸·ÎºÎÅÍ ºÐ¸®ÇÑ ºñ¼ö¿ë¼º ±Û·çĵ Çü¼º ¾ïÁ¦¼¼±ÕÀÇ È¿°ú¸¦ ºñÄ¿ ¿ÍÀÌ¾î °Ë»ç·Î º» °á°ú, ±³Á¤¿ë ¿ÍÀ̾î»ó¿¡ Çü¼ºµÈ Ä¡ÅÂÀÇ ¹«°Ô°¡ Streptococcus mutans ´Üµ¶¹è¾ç½Ã 152mg¿¡¼­ Enterococcus durans, Lactobacillus acidophilus, Streptococcus oralis¿ÍÀÇ È¥ÇÕ¹è¾ç½Ã °¢°¢ 4mg, 78mg, 72mgÀ¸·Î °¨¼ÒµÇ¾ú´Ù. Streptococcus mutansÀÇ »ý±Õ¼ö´Â ´Üµ¶¹è¾ç½Ã ml´ç 3.6¡¿ lO^(8) °³¿¡¼­ Enterococcus durans, Lactobacillus acidophilus, Streptococcus oralis¿ÍÀÇ È¥ÇÕ¹è¾ç½Ã °¢°¢ ml´ç 1.4¡¿10^(6), 5.6¡¿10^(6), 3.8¡¿10^(6)À¸·Î °¨¼ÒÇÏ¿´´Ù. ¼Ò¾Æ·ÎºÎÅÍ ¾òÀº Ÿ¾×À» BHI agar¿¡ Á¢Á¾ÇÏ¿´À» ¶§ÀÇ »ý±Õ¼ö´Â 4.8¡¿10^(6)¿¡¼­ 1.3¡¿10^(9)À̾úÀ¸³ª, ÀÌ·¯ÇÑ Àüü ¼¼±ÕÀÇ
³óµµ´Â Ÿ¾×³» Enterococcus, Lactobacillus ¸¦ ¾ïÁ¦ÇÏ´Â StreptococcusÀÇ ³óµµ¿Í °ü·ÃÀÌ ¾ø¾ú´Ù.
ÀÌ»óÀÇ °á°ú¸¦ Á¾ÇÕÇÏ¸é ¼Ò¾ÆÀÇ ±¸°­À¸·ÎºÎÅÍ ºÐ¸®ÇÑ ºñ¼ö¿ë¼º ±Û·çĵ Çü¼º ¾ïÁ¦ ¼¼±ÕÀÇ Streptococcus mutans Ä¡ÅÂÇü¼º¿¡ ´ëÇÑ ¾ïÁ¦´Â Streptococcus mutansÀÇ Áõ½ÄÀ» ¾ïÁ¦ÇÏ¿© ÀϾÀ¸¸ç, Enterococcus, Lactobacillus, Streptococcus mutans¸¦ ¾ïÁ¦ÇÏ´Â StreptococcusÀÇ ±¸°­³» ³óµµ´Â ±¸°­ Áï Ÿ¾×³» Àüü ¼¼±ÕÀÇ ³óµµ¿¡ ¿µÇâÀ» ¹ÌÄ¡Áö ¾Ê¾Ò´Ù.
The inhibition degree of the isolated bacteria on plaque formation of Streptococcus mutans, and the effect of these bacterial genus on the concentration of total bacteria in saliva were assessed with the following.
The effectiveness of the isolated bacteria on the inhibition of plaque formation was assessed culturing Streptococcus mutans in the beaker with orthodontic wires. The mean weight of plaque produced on a wire was 152mg in the culture of Streptococcus mutans only, whereas being reduced to 4 mg, 78mg, or 72rng in the combined culture of Streptococcus mutans and Enterococcus durans, Lactobacillus acidophilus, or Streptococcus oralis, The colony forming units (CFU) of Streptococcus mutans were 3.6 x 108 per ml in the culture of Streptococcus mutans, only, wheras being 1.4 x 106, 5.6 x 106, or 3.8 x 106 per ml in the combined culture of Streptococcus mutans and Enterococcus durans, Lactobacillus acidophilus, or Streptococcus oralis. When saliva from children was inoculated on brain heart infusion agar, the colony forming units of bacteria were 4.8 x 106 to 1.3 x 109 per ml of saliva. The concentration of Enterococcus, Lactobacillus, or Streptococcus inhibiting Streptococcus mutans in saliva was not proportioned to that of total bacteria replicated on brain heart infusion agar.
These results indicate that the isolated bacteria inhibited the replication of Streptococcus mutans, resulting into inhibiting the formation of plaque, but the concentration of Enterococcus, Lactobacillus, or Streptococcus inhibiting Streptococcus mutans, in saliva might not affect the total bacterial concentration of saliva.

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Ä¡ÅÂ;Streptococcus mutans;Plaque;Streptococcus mutans

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